Stress and the Doggy Gut: Microbiome Differences

Dogs with anxiety or aggression showed a distinct gut bacterial profile compared to controls.

Journal: Scientific Reports
Sample Size: 50 dogs (25 high-anxiety/aggression, 25 controls)
Study Type: Cross-sectional cohort microbiome analysis
Published: 2025
Species:

Key Findings

  • Distinct gut bacterial composition in anxious/aggressive dogs
  • Lower Faecalibacterium abundance in high-anxiety group
  • Higher Blautia in anxious profiles
  • Supports gut-brain axis relevance
  • Potential future probiotic targets

Introduction

Many dog owners know that stress and anxiety can affect their pet’s behavior, but fewer realize these feelings may also shape their dog’s gut health. Understanding the link between a dog’s mood and their digestive system opens new doors for supporting both emotional and physical well-being.

Research Background

Canine anxiety and aggression are common behavioral concerns that impact quality of life for pets and families. While behavior modification and medication are standard approaches, recent research suggests the gut microbiome—an ecosystem of bacteria in the intestines—may also play a role in emotional regulation. However, until now, specific differences in gut bacteria between anxious/aggressive dogs and their calmer counterparts have not been fully characterized.

Study Details

  • Researchers conducted a cross-sectional cohort analysis with 50 dogs: 25 identified as high-anxiety/aggression and 25 as controls.
  • Dogs were assessed using behavioral scoring systems to determine group assignment.
  • Fecal samples were collected and analyzed via 16S rRNA sequencing to profile gut bacteria.
  • The study compared bacterial diversity and abundance between the two groups.

Key Findings

Primary Results

  • Dogs with anxiety or aggression showed a distinct gut bacterial profile compared to controls.
  • Faecalibacterium, a group of bacteria often associated with gut health, was significantly reduced in the high-anxiety/aggression group.
  • Blautia levels were elevated in anxious/aggressive dogs, a pattern not observed in the control group.
  • These differences support the relevance of the gut-brain axis—how gut health may influence behavior and vice versa.

Secondary Findings

  • Reduced abundance of beneficial bacteria in anxious/aggressive dogs may be linked to inflammatory processes.
  • The findings highlight potential targets for future probiotic or dietary interventions aimed at supporting both gut and behavioral health.

Implications for Pet Owners

What This Means for You

  • Behavior and gut health are connected: If your dog struggles with anxiety or aggression, their digestive health may also be affected. Supporting the gut could help complement traditional behavioral strategies.
  • Consider a holistic approach: Managing canine anxiety might benefit from integrating dietary modifications, probiotics, and behavioral therapies.
  • Monitor dietary choices: Diets that promote a healthy balance of gut bacteria—such as those rich in fiber—may be helpful. Discuss options with your veterinarian.
  • Probiotics are promising but not yet proven: While this study points to certain bacteria as targets for future probiotics, there is not enough evidence to recommend specific products for anxiety at this time.
  • Watch for digestive signs: Dogs with anxiety may also show changes in appetite, stool consistency, or gastrointestinal discomfort.

When to Consult Your Veterinarian

  • If your dog’s anxiety or aggression is worsening or interfering with daily life.
  • If you notice persistent gastrointestinal symptoms (vomiting, diarrhea, constipation, or dramatic stool changes).
  • Before starting any new diet, supplement, or probiotic.
  • If behavioral interventions alone are not improving your dog’s quality of life.

Study Limitations

  • Cross-sectional design cannot establish cause and effect.
  • Modest sample size (50 dogs) limits generalizability.
  • Only fecal bacteria analyzed; other gut factors not measured.
  • Behavioral assessments may vary between observers.
  • Results may not apply to all breeds or environments.

Bottom Line

Supporting your dog’s gut health may be an important part of managing anxiety and aggression. Work with your veterinarian to explore the best combination of behavioral and nutritional strategies for your pet.

Quick Action Checklist

  • Observe your dog’s behavior and note any signs of stress or aggression.
  • Monitor for digestive changes such as stool consistency or appetite loss.
  • Discuss gut health and behavioral concerns with your veterinarian.
  • Consider dietary options that promote healthy gut bacteria (fiber-rich foods, as recommended).
  • Avoid making sudden changes to your dog’s diet or supplements without professional advice.
  • Stay updated on research about probiotics and canine anxiety.
  • Combine behavioral training with holistic health approaches.
  • Seek prompt veterinary care for severe anxiety or persistent digestive issues.

Disclaimer

This research brief is intended for educational purposes only and does not replace professional veterinary advice. If your dog is experiencing anxiety, aggression, or digestive problems, consult your veterinarian for personalized recommendations and care.

Reference

Not specified. "Stress and the Doggy Gut: Microbiome Differences". Scientific Reports. 2025. DOI: 10.1038/s41598-025-06178-4